Insects in the field are exposed to multiple bouts of cold, and there is increasing evidence that the fitness consequences of repeated cold exposure differ from the impacts of a single cold exposure. We tested the hypothesis that different kinds of cold exposure (in this case, single short, prolonged and repeated cold exposure) would result in differential gene expression. We exposed 3 day old adult female wild-type Drosophila melanogaster (Diptera: Drosophilidae) to –0.5°C for a single 2 h exposure, a single 10 h exposure, or five 2 h exposures on consecutive days, and extracted RNA after 6 h of recovery. Global gene expression was quantified using an oligonucleotide microarray and validated with real-time PCR using different biological replicates. We identified 76 genes upregulated in response to multiple cold exposure, 69 in response to prolonged cold exposure and 20 genes upregulated in response to a single short cold exposure, with a small amount of overlap between treatments. Three genes – Turandot A, Hephaestus and CG11374 – were upregulated in response to all three cold exposure treatments. Key functional groups upregulated include genes associated with muscle structure and function, the immune response, stress response, carbohydrate metabolism and egg production. We conclude that cold exposure has wide-ranging effects on gene expression in D. melanogaster and that increased duration or frequency of cold exposure has impacts different to those of a single short cold exposure. This has important implications for extrapolating laboratory studies of insect overwintering that are based on only a single cold exposure.
The Oct. 20, article [“New Schenectady pump station plan unveiled”] about the revised design and location of the new pump station in Riverside Park included a couple of statements that need clarification.First, the new design features rectangular windows, not arched ones as stated in the article. The state Historic Preservation Office worked with the consultants to design a new building that will look distinct from the existing station with its arched windows.Second, the original proposed location was not at a higher elevation. It would have taken up a large portion of the west side of the park, all of which is at the same elevation. The new design will take a much smaller portion of the park and leave the west side intact.Suzanne UngerSchenectadyThe writer is the recording secretary for the Stockade Association.More from The Daily Gazette:Anderson starts, but Dodgers finish off NLCS winSchenectady, Saratoga casinos say reopening has gone well; revenue down 30%Motorcyclist injured in Thursday afternoon Schenectady crashSchenectady police reform sessions pivot to onlineSchenectady NAACP calls for school layoff freeze, reinstatement of positions Categories: Letters to the Editor, OpinionClearing up errors about pump station
Charlie’s became a staple of Gold Coast life in the ’70s and ’80s where families would flock during the day and shift workers, taxi drivers and party animals would be well catered for at night. Mr Wriggles died of a heart attack in the early ’80s and Mrs Wriggles took over running the business and became a popular fixture at the restaurant. She died in August, aged 90. Gold Coast stalwart Rita Wriggles was best known for owning Charlie’s in Surfers Paradise from the early 80s to 1995. The Wriggles owned the home at 7 Valencia Ave, Isle of Capri, for more than 40 years.Her family have made the difficult decision to sell the three-bedroom family home at 7 Valencia Ave for the first time in its history. Graham Wriggles, the son of Rita and Brian, said the house had been in the family for more than 40 years and he would be sad to see it go. “Dad retired from the navy in the 1970s and went back to his beloved Queensland and that’s when he bought the property,” he said. “Mum loved the fishing and dad loved his boat and it was close to Surfers.“They started Charlie’s in 1977, turned it from a fairly nice coffee shop into a 24-hour ripper.” It’s the first time its been up for sale since it was built.More from news02:37International architect Desmond Brooks selling luxury beach villa9 hours ago02:37Gold Coast property: Sovereign Islands mega mansion hits market with $16m price tag1 day ago The waterfront house offers three bedrooms, two bathrooms and a pool.The property is listed with an expressions-of-interest campaign closing on December 4 through Lucy Cole and her self-titled agency. “It’s original condition but has been all tarted up,” Ms Cole said. “They (Rita and Brian) bought it off the builder. It was one of the first homes on the Isle of Capri. “The reason they chose Isle of Capri was its proximity to the cafe, beach and river. It’s a sought-after location.”Ms Cole said the property was likely to attract a range of buyers including land bankers, investors, holiday-makers, renovators or those looking for a new home site. Video Player is loading.Play VideoPlayNext playlist itemMuteCurrent Time 0:00/Duration 1:27Loaded: 0%Stream Type LIVESeek to live, currently playing liveLIVERemaining Time -1:27 Playback Rate1xChaptersChaptersDescriptionsdescriptions off, selectedCaptionscaptions settings, opens captions settings dialogcaptions off, selectedQuality Levels720p720pHD540p540p360p360p270p270pAutoA, selectedAudio Tracken (Main), selectedFullscreenThis is a modal window.Beginning of dialog window. Escape will cancel and close the window.TextColorWhiteBlackRedGreenBlueYellowMagentaCyanTransparencyOpaqueSemi-TransparentBackgroundColorBlackWhiteRedGreenBlueYellowMagentaCyanTransparencyOpaqueSemi-TransparentTransparentWindowColorBlackWhiteRedGreenBlueYellowMagentaCyanTransparencyTransparentSemi-TransparentOpaqueFont Size50%75%100%125%150%175%200%300%400%Text Edge StyleNoneRaisedDepressedUniformDropshadowFont FamilyProportional Sans-SerifMonospace Sans-SerifProportional SerifMonospace SerifCasualScriptSmall CapsReset restore all settings to the default valuesDoneClose Modal DialogEnd of dialog window.This is a modal window. This modal can be closed by pressing the Escape key or activating the close button.Close Modal DialogThis is a modal window. This modal can be closed by pressing the Escape key or activating the close button.PlayMuteCurrent Time 0:00/Duration 0:00Loaded: 0%Stream Type LIVESeek to live, currently playing liveLIVERemaining Time -0:00 Playback Rate1xFullscreenWhy Spring 2019 is a good time to sell01:27 The former family home of Rita and Brian Wriggles, the couple who started Charlie’s Restaurant Bar and Cafe, has been listed for sale.THE former home of the couple behind one of Surfers Paradise’s most famed restaurants has hit the market. The Isle of Capri house was purchased by Rita and Brian Wriggles in the 1970s before they opened what would become a Gold Coast institution – Charlie’s Restaurant Bar and Cafe. MORE NEWS: A Queenslander that might be your cup of tea MORE NEWS: International buyers splash big cash on rainforest estate
Statewide—On Friday, Governor Eric J. Holcomb signed Executive Order 20-24 to allow health care providers and facilities to resume elective medical procedures provided they have sufficient quantities of Personal Protective Equipment (PPE) and consult the best practices and recommendations developed by their medical associations or industries.Providers include hospitals, veterinarians, dentists, and others listed in Executive Order.